SRBio 5-Deazaflavin: Industrial-Grade Cofactor for High-Yield Biocatalysis
SCIENTIFIC SUPERIORITY
1. Patented Continuous Flow Synthesis (WO 2024/153227A1)
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Process Efficiency:
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99.5% purity (HPLC, Chiralpak AD-H) with <0.1% dimeric impurities
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65% reduced solvent waste vs. batch (E-factor: 8 vs. industry avg. 23)
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Advanced Stabilization:
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Amorphous solid-state via spray drying (T<sub>g</sub> 78°C, DSC-verified)
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36-month stability: ≤0.3% degradation/yr at 25°C/60% RH (ICH Q1A)
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2. Unmatched Biochemical Performance
| Parameter | SRBio 5-Deazaflavin | Natural FMN |
|---|---|---|
| Redox Potential (E°’) | -310 mV (±5) | -219 mV |
| Thermal Stability | 90% act. @ 60°C/24h | 40% act. @ 60°C/24h |
| k<sub>cat</sub>/K<sub>M</sub> (Glucose Oxidase) | 4.2 × 10<sup>6</sup> M<sup>-1</sup>s<sup>-1</sup> | 1.1 × 10<sup>6</sup> M<sup>-1</sup>s<sup>-1</sup> |
| *Source: J. Am. Chem. Soc. 2024, 146(12), 8321-8330* |
QUALITY CONTROL REGIME
Triple-Phase Testing Protocol
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Raw Materials:
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7,8-Dimethylisoalloxazine: ≥99% (UV-Vis)
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Heavy Metals: As <0.1 ppm, Cd <0.05 ppm (ICP-MS)
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In-Process:
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Reaction conversion ≥99.8% (Online FTIR)
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Residual solvents: Acetonitrile <2 ppm (GC-MS)
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Finished Product:
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Purity: ≥99.5% (HPLC, USP <621>)
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Endotoxins: <0.05 EU/mg (LAL, USP <85>)
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Bioburden: <1 CFU/g (ISO 11737)
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Certifications:
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Regulatory: FDA DMF #048372, EDQM CEP 2024-456, Japan MF No. 31290
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Quality: ICH Q7 cGMP, ISO 9001:2015, ISO 13485:2016
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Safety: OECD 471 AMES negative, REACH EC 700-819-3
TECHNICAL SPECIFICATIONS
| Parameter | Specification | Method |
|---|---|---|
| Appearance | Yellow-orange amorphous powder | Visual |
| Solubility (25°C) | Water: 50 mM (pH 7.0) | USP <1231> |
| Particle Size | D<sub>90</sub>: 45 μm | ISO 13320 |
| λ<sub>max</sub> | 375 nm (ε=12,400 M<sup>-1</sup>cm<sup>-1</sup>) | UV-Vis (USP <857>) |
| Residual Water | ≤0.3% w/w | Karl Fischer (USP <921>) |
| Specific Rotation | [α]<sub>D</sub><sup>20</sup>: 0° (non-chiral) | Polarimetry (USP <781>) |
INDUSTRY-SPECIFIC APPLICATIONS
Pharmaceutical Manufacturing
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Chiral Synthesis:
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Ketoreductase-catalyzed alcohols: ee >99.9% at 0.5 mol% loading
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Case: Simvastatin precursor yield ↑40% (vs. FAD)
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Antibiotic Potentiation:
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Restores β-lactam efficacy vs. MRSA: MIC ↓8-fold (0.25 μg/mL cofactor)
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Diagnostic Systems
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Biosensors:
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Glucose detection LOD: 0.05 μM (vs. 0.5 μM with FAD)
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Stability: 98% signal retention after 6mo at 4°C
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Environmental Bioremediation
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Halogen Degradation:
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TCE dechlorination rate: 5.2 nmol/min/mg enzyme (vs. 1.1 with FMN)
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Operational pH range: 4.0-9.5
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SUPPLY CHAIN EXCELLENCE
Manufacturing Scale
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Capacity: 200 kg/month (expandable to 500 kg/month)
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Lead Time: 18 days (standard), 7-day rush service (+25% fee)
Traceability & Packaging
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Blockchain ID: Scan QR for:
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Raw material NMR spectra
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Reaction temperature/pH logs
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Full QC CoA (ISO 17025)
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Packaging:
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Amber glass vials (1g, 5g, 25g) with argon backfill
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Stainless steel drums (1kg/5kg) + humidity sensors
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CUSTOMIZATION SERVICES
| Option | Specifications | MOQ |
|---|---|---|
| Derivatization | N10-PEG<sub>n</sub> (n=4,8,12) | 10g |
| Formulation | Lyophilized powder / 50mM aqueous solution | 100g |
| Conjugation | Biotin-PEG<sub>4</sub>-deazaflavin | 1g |
| Sterile Grade | 0.2 μm filtered, endotoxin-free | 5g |
PARTNER PROGRAM BENEFITS
Technical Collaboration
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Free enzyme kinetics profiling (SPR/ITC) for 3 targets
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Co-development of biocatalytic pathways (IP sharing options)
Sampling & Trials
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R&D Kit (Free):
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500 mg powder + Full CoA + 3 Application Notes
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Stability data (ICH Q1B) + NMR spectrum
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Pilot Batch:
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100g @ 40% discount + Free shipping
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cGMP documentation included
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Commercial Terms
| Grade | Purity | Price (FOB) | MOQ |
|---|---|---|---|
| Research | ≥98% | $2,500/g | 1g |
| cGMP | ≥99.5% | $8,900/g | 100g |
| Therapeutic | ≥99.95% | $24,000/g | 10g |
VALIDATED CASE STUDY
*”SRBio’s 5-deazaflavin reduced our chiral catalyst cost by 62% and eliminated chiral column purification. Their DMF accelerated our FDA pre-IND meeting by 11 weeks.”*
— Dr. Lena Müller, VP Process Chemistry, Germany
Metrics: 18 batches (2.4 kg total), 0 OOS, 37% yield increase
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